Search results for "DNA single strand"

showing 4 items of 4 documents

Increasing rates of DNA single-strand breaks in lymphocytes of clinical personnel handling cytostatic drugs

1993

A total of 27 persons, working in cancer stations with exposure to cytostatics, and 40 healthy control persons were examined for DNA single-strand breaks in peripheral lymphocytes. Non-smoking personnel from cancer stations were found to have an increased rate of DNA single-strand breaks compared to the non-smoking control subjects. In the case of smokers an increased rate of DNA single-strand breaks could be recorded for those working in cancer stations as well as with the controls. DNA single-strand breaks indicate reversible damage to DNA. As DNA repair is not perfect in every case, an increased number of DNA single-strand breaks leads to irreversible DNA damage.

AdultMaleCancer Researchmedicine.medical_specialtyDNA damageDNA repairLymphocyteDNA Single-StrandedAntineoplastic AgentsBiologyMedical Oncologychemistry.chemical_compoundInternal medicinemedicineHumansLymphocytesGeneticsDNA single strandHematologyCancerGeneral MedicineMiddle Agedmedicine.diseasePersonnel Hospitalmedicine.anatomical_structureOncologychemistryToxicityCancer researchFemaleDNADNA DamageJournal of Cancer Research and Clinical Oncology
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DNA single strand break analysis in mononuclear blood cells of petrol pump attendants

1995

DNA single strand breaks, including DNA adducts that lead to alkali-labile sites, were measured in peripheral mononuclear blood cells of 35 petrol pump attendants by alkaline filter elution. Blood samples from petrol pump attendants were taken on Monday and Friday. Additionally, DNA single strand breaks of smoking and non-smoking control persons were examined. For the smoking (n = 12) and the non-smoking controls (n = 20) a mean normalized elution rate of 1.49 +/- 0.52 (mean value +/- 95% confidence interval) and 1.32 +/- 0.28, respectively, was obtained. The difference between smoking and non-smoking controls was not statistically significant (U test). An increase in DNA single strand brea…

AdultMaleeducationAnimal scienceCigarette smokingRisk FactorsOccupational ExposureHumansMedicineDNA Single Strand BreakDNA single strandElution ratebusiness.industryOrganic solventSmokingMean valuePublic Health Environmental and Occupational HealthDNAMiddle AgedConfidence intervalPetroleumLeukocytes MononuclearOccupational exposurebusinesshuman activitiesDNA DamageEnvironmental MonitoringInternational Archives of Occupational and Environmental Health
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In vivo ozone exposure does not increase DNA single-strand breaks in human peripheral lymphocytes

2013

In this randomized parallel study, we examined whether an acute ozone (O3) exposure leads to increased DNA strand breaks in human lymphocytes. The groups were exposed to 0.21 ppm O3 or filtered air for two hours. 30min and 4.5 h after exposure, DNA damage was determined in isolated lymphocytes using the Fast Micromethod. There was no detectable effect after O3 exposure. We conclude that an acute O3 exposure at the tested concentration does not lead to persistent DNA damage.

DNA single strandOzoneDNA damageHealth Toxicology and MutagenesisDNA Single-StrandedParallel studyGeneral MedicineBiologyToxicologyMolecular biologyPeripheralchemistry.chemical_compoundOzonechemistryIn vivoImmunologyHumansLymphocytesOzone exposureDNADNA DamageHuman & Experimental Toxicology
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Survival in extreme dryness and DNA-single-strand breaks.

1992

A wide variety of organisms (the so-called "anhydrobiotes') is able to survive long periods of time in a state of utmost dehydration and can thus survive in extremely dry environments including artificially imposed or space vacuum. Known strategies of survival include the accumulation of certain polyols, especially disaccharides, which help prevent damage to membranes and proteins. Here we report that DNA in vacuum-dried spores is damaged to a very substantial degree by processes leading to DNA strand breaks. Most of these lesions are obviously repaired during germination, but extensive damage to DNA and enzymes after long exposure times (months to years) finally diminish the chances of sur…

DNA BacterialAtmospheric ScienceDNA RepairVacuumDNA damageDNA repairAerospace EngineeringGerminationBiologyAgar gelchemistry.chemical_compoundmedicineDesiccationDNA single strandElectrophoresis Agar GelSpores BacterialAstronomy and AstrophysicsCell biologyGeophysicschemistrySpace and Planetary ScienceGeneral Earth and Planetary SciencesDrynessAutoradiographymedicine.symptomDesiccationDNABacillus subtilisDNA DamageAdvances in space research : the official journal of the Committee on Space Research (COSPAR)
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